Generation of a gene-corrected human iPSC line (CSUASOi004-A-1) from a retinitis pigmentosa patient with heterozygous c.2699 G>A mutation in the PRPF6 gene

Yuqin Liang; Xihao Sun; Chunwen Duan; Yalan Zhou; Zekai Cui; Chengcheng Ding; Jianing Gu; Shengru Mao; Shangli Ji; Hon Fai Chan; Shibo Tang; Jiansu Chen

Stem Cell Research (Oct 2022)

Generation of a gene-corrected human iPSC line (CSUASOi004-A-1) from a retinitis pigmentosa patient with heterozygous c.2699 G>A mutation in the PRPF6 gene

Yuqin Liang,
Xihao Sun,
Chunwen Duan,
Yalan Zhou,
Zekai Cui,
Chengcheng Ding,
Jianing Gu,
Shengru Mao,
Shangli Ji,
Hon Fai Chan,
Shibo Tang,
Jiansu Chen

Affiliations

Yuqin Liang: Aier School of Ophthalmology, Central South University, P.R.C., 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Aier Eye Institute, 18th Floor, The New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China
Xihao Sun: Aier School of Ophthalmology, Central South University, P.R.C., 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Aier Eye Institute, 18th Floor, The New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China
Chunwen Duan: Aier School of Ophthalmology, Central South University, P.R.C., 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Aier Eye Institute, 18th Floor, The New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China
Yalan Zhou: Aier School of Ophthalmology, Central South University, P.R.C., 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Aier Eye Institute, 18th Floor, The New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China
Zekai Cui: Aier Eye Institute, 18th Floor, The New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China
Chengcheng Ding: Aier Eye Institute, 18th Floor, The New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China
Jianing Gu: Aier Eye Institute, 18th Floor, The New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China
Shengru Mao: Aier Eye Institute, 18th Floor, The New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China
Shangli Ji: Aier Eye Institute, 18th Floor, The New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China
Hon Fai Chan: Institute for Tissue Engineering and Regenerative Medicine, The Chinese University of Hong Kong, 999077, Hong Kong, China; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, 999077, Hong Kong, China
Shibo Tang: Aier School of Ophthalmology, Central South University, P.R.C., 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Aier Eye Institute, 18th Floor, The New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China; CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China; Corresponding authors at: Aier School of Ophthalmology, Central South University, P.R.C., 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China.
Jiansu Chen: Aier School of Ophthalmology, Central South University, P.R.C., 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Aier Eye Institute, 18th Floor, The New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Key Laboratory of Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, China; Corresponding authors at: Aier School of Ophthalmology, Central South University, P.R.C., 18th Floor, the New Century Building, #198 Furong Middle Road, Changsha, Hunan 410015, China.

Journal volume & issue: Vol. 64
p. 102911

Abstract

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Retinitis pigmentosa (RP) is one of the most common inherited retinal diseases characterized by nyctalopia, progressive vision loss and visual field contraction. we previously generated an induced pluripotent stem cell line (CSUASOi004-A) from a RP patient with heterozygous PRPF6 c.2699 G>A (p.R900H) mutation. Here we corrected the PRPF6 c.2699 G>A mutation genetically using CRISPR/Cas9 technology to generate an isogenic control (CSUASOi004-A-1), which can provide a valuable resource in the research of the disease.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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