Human Chr18 transcriptome dataset combined from the Illumina HiSeq, ONT MinION, and qPCR data
George Krasnov,
Timur Shkrigunov,
Sergey Radko,
Konstantin Ptitsyn,
Valeriya Shapovalova,
Olga Timoshenko,
Svetlana Khmeleva,
Leonid Kurbatov,
Yana Kiseleva,
Ekaterina Ilgisonis,
Olga Kiseleva,
Igor Vakhrushev,
Anastasia Tsvetkova,
Ivan Buromski,
Sergey Markin,
Alexander Archakov,
Andrey Lisitsa,
Elena Ponomarenko
Affiliations
George Krasnov
Institute of Biomedical Chemistry, Moscow, Russia; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia; Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
Timur Shkrigunov
Institute of Biomedical Chemistry, Moscow, Russia; Corresponding author.
Sergey Radko
Institute of Biomedical Chemistry, Moscow, Russia
Konstantin Ptitsyn
Institute of Biomedical Chemistry, Moscow, Russia
Valeriya Shapovalova
Centre for Strategic Planning and Management of Biomedical Health Risks, Federal Medical Biological Agency, Moscow, Russia
Olga Timoshenko
Institute of Biomedical Chemistry, Moscow, Russia
Svetlana Khmeleva
Institute of Biomedical Chemistry, Moscow, Russia
Leonid Kurbatov
Institute of Biomedical Chemistry, Moscow, Russia
Yana Kiseleva
Russian Scientific Center of Roentgen Radiology, Moscow, Russia
Ekaterina Ilgisonis
Institute of Biomedical Chemistry, Moscow, Russia
Olga Kiseleva
Institute of Biomedical Chemistry, Moscow, Russia
Igor Vakhrushev
Institute of Biomedical Chemistry, Moscow, Russia
Anastasia Tsvetkova
Institute of Biomedical Chemistry, Moscow, Russia
Ivan Buromski
Department of Forensic Medicine, Faculty of General Medicine, Pirogov Russian National Research Medical University, Moscow, Russia
The chromosome-centric dataset was created by applying several technologies of transcriptome profiling. The described dataset is available at NCBI repository (BioProject ID PRJNA635536). The dataset referred to the same type of tissue, cell lines, transcriptome sequencing technologies, and was accomplished in a period of 8 years (the first data were obtained in 2013 while the last ones — in 2020). The high-throughput sequencing technologies were employed along with the quantitative PCR (qPCR) approach, for data generation using the gene expression level assessment. qPCR was performed for a limited group of genes, encoded on human chromosome 18, for the Russian part of the Chromosome-Centric Human Proteome Project. The data of high-throughput sequencing are provided as Excel spreadsheets, where the data on FPKM and TMP values were evaluated for the whole transcriptome with both Illumina HiSeq and Oxford Nanopore Technologies MinION sequencing.
