Antimicrobial Properties of Water-Soluble Polysaccharides and Alcoholic Extracts of <i>Laetiporus Sulphureus</i> (Bull.) Murrill Mycelium and Development of Biotechnology for Its Production in Immobilized Culture on Bacterial Cellulose

Abstract

The article discusses the development of a new method of producing Laetiporus sulphureus (Bull.) Murrill basidiomycete mycelium immobilized on a bacterial cellulose matrix. Mycelium contains biologically active compounds with antimicrobial activity against Gram-positive bacteria, including resistant staphylococcus. The aim of the work was to obtain immobilized mycelium by co-cultivation of L.sulphureus with the producer of bacterial cellulose Gluconacetobacter hansenii. The authors found that when co-culturing the basidial L.sulphureus strain with the bacterial cellulose producing G.hansenii strain, productivity increases by 3.2 times on H5/1 synthetic medium and by 1.9 times on natural Maltax-10 medium (concentration 5%). The resulting immobilized L.sulphureus mycelium has antibacterial properties; its aqueous extracts contain glucans.

Keywords

• <i>laetiporus sulphureus</i>
• xylotrophic basidiomycete
• biotechnology
• immobilized mycelium
• bacterial cellulose
• antimicrobial activity
• <i>gluconacetobacter hansenii</i>