Guangxi Zhiwu (Feb 2023)
Identification and analysis of TCP transcription factors in response to low nitrogen fertilizer stress in Solanum tuberosum
Abstract
TCP transcription factors are a type of plant-specific transcription factors, which have been implicated in multiple aspects of plant biological processes. To study the role of potato TCP transcription factors in response to low nitrogen fertilizer stress, here, four transcriptome libraries from potato roots and leaves under the conditions of low nitrogen fertilizer (0.05 mmol·L-1) and sufficient nitrogen fertilizer (7.5 mmol·L-1) were constructed for transcriptome sequencing, respectively. Meanwhile, the differentially expressed TCP transcription factor were analyzed. The results were as follows: (1) A total of 24 TCP transcription factors were identified in the four transcriptome libraries, and mainly distributed on the second, third and sixth chromosomes. (2) The analysis of domains showed that all of 24 transcription factors contained basic-Helix-Loop-Helix domain. (3) The phylogenetic analysis showed that the TCP proteins of potato and Arabidopsis thaliana were closely related, and clustered into ten subgroups. (4) Transcriptome sequencing results showed that the expression levels of most potato TCP transcription factors were suppressed by low nitrogen fertilizer stress. Among them, three TCP transcription factors were significantly differentially expressed in roots, while five TCP transcription factors were specifically expressed in leaves. (5) According to the GO functional analysis and the relationship between the potato and the A. thaliana TCP transcription factors, it is predicted that these TCP transcription factors were involved in response of potato to low nitrogen fertilizer stress. The research provides a foundation for further study on the molecular role of transcription factors in response to low nitrogen fertilizer stress in potato and other crops.
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