International Journal of Nanomedicine (Oct 2020)
Clinical Application for Screening Down’s Syndrome by Using Carboxylated Graphene Oxide-Based Surface Plasmon Resonance Aptasensors
Abstract
Nan-Fu Chiu,1,2 Ying-Hao Wang,1,3 Chen-Yu Chen3,4 1Laboratory of Nano-Photonics and Biosensors, Institute of Electro-Optical Engineering, National Taiwan Normal University, Taipei City 11677, Taiwan; 2Department of Life Science, National Taiwan Normal University, Taipei City 11677, Taiwan; 3Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei City 10449, Taiwan; 4Department of Medicine, Mackay Medical College, Taipei City 25245, TaiwanCorrespondence: Nan-Fu ChiuLaboratory of Nano-photonics and Biosensors, Institute of Electro-Optical Engineering, National Taiwan Normal University, No. 88, Sec. 4, Ting-Chou Road, Taipei 11677, TaiwanTel +886-2-77496722Fax +886-2-86631954Email [email protected] ChenDepartment of Obstetrics and Gynecology, Mackay Memorial Hospital, No. 92, Sec. 2, Zhongshan N. Road, Taipei City 10449, TaiwanTel +886-2-25433535Fax +886-2-25433642Email [email protected]: Advanced medical detection technology requires high sensitivity and accuracy to increase the disease detection rate. We showed that carboxyl-functionalized graphene oxide (carboxyl-GO) biosensing materials are capable of accurate detection.Methods: We developed a carboxylated GO-based surface plasmon resonance (SPR) aptasensor suitable for screening Down’s syndrome in clinical serum. This biosensing material could rapidly and accurately detect hCG protein with a low concentration to identify fetal Down’s syndrome. The developed carboxyl-GO-based SPR aptasensor showed excellent sensitivity and limit of detection without the use of antibodies and without any specific preference.Results: hCG protein detection limits of 1 pM in buffer samples and 1.9 pM in clinical serum samples were achieved. The results showed that the carboxyl-GO-based chip could detect hCG well below the normal physiological level of serum protein (5.0 mIU/mL). High affinity, sensitivity, and better detection limit were obtained in the range of 1.9 pM to 135 pM. The results showed a 5k-fold dilution factor, and that an SPR angle shift of more than 20 millidegrees (mo) was associated with a significant risk of fetal Down’s syndrome compared to normal pregnant women. The results clearly showed that the detection of hCG protein in serum samples from pregnant women at 12– 19 weeks could be used to screen Down’s syndrome with high selectivity and sensitivity.Conclusion: Our findings suggest the potential application of carboxyl-GO film in proof-of-concept studies for serum assays as a new type of SPR material. In addition, peptide and carboxyl-GO films may be conducive to the development of future point of care testing and rapid diagnostic devices for other diseases such as cancer.Keywords: surface plasmon resonance, SPR, aptasensor, carboxyl-functionalized graphene oxide, GO-COOH, peptides, human chorionic gonadotropin, hCG, Down’s syndrome, DS
