Whole cells of recombinant CYP153A6-E. coli as biocatalyst for regioselective hydroxylation of monoterpenes

Pietro Cannazza; Marco Rabuffetti; Silvia Donzella; Valerio De Vitis; Martina L. Contente; Maria da Conceição Ferreira de Oliveira; Marcos C. de Mattos; Francisco G. Barbosa; Ricardo Pinheiro de Souza Oliveira; Andrea Pinto; Francesco Molinari; Diego Romano

doi:10.1186/s13568-022-01389-8

AMB Express (Apr 2022)

Whole cells of recombinant CYP153A6-E. coli as biocatalyst for regioselective hydroxylation of monoterpenes

Pietro Cannazza,
Marco Rabuffetti,
Silvia Donzella,
Valerio De Vitis,
Martina L. Contente,
Maria da Conceição Ferreira de Oliveira,
Marcos C. de Mattos,
Francisco G. Barbosa,
Ricardo Pinheiro de Souza Oliveira,
Andrea Pinto,
Francesco Molinari,
Diego Romano

Affiliations

Pietro Cannazza: Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan
Marco Rabuffetti: Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan
Silvia Donzella: Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan
Valerio De Vitis: Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan
Martina L. Contente: Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan
Maria da Conceição Ferreira de Oliveira: Laboratory of Biotechnology and Organic Synthesis (LABS), Department of Organic and Inorganic Chemistry, Science Centre, Federal University of Ceará
Marcos C. de Mattos: Laboratory of Biotechnology and Organic Synthesis (LABS), Department of Organic and Inorganic Chemistry, Science Centre, Federal University of Ceará
Francisco G. Barbosa: Laboratory of Biotechnology and Organic Synthesis (LABS), Department of Organic and Inorganic Chemistry, Science Centre, Federal University of Ceará
Ricardo Pinheiro de Souza Oliveira: Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo
Andrea Pinto: Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan
Francesco Molinari: Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan
Diego Romano: Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan

DOI: https://doi.org/10.1186/s13568-022-01389-8
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 9

Abstract

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Keypoints Different monoterpenes can be regioselectively hydroxylated by CYP153A6 monooxygenase The biotransformation with whole cells is complementary to chemical oxyfunctionalization Fed-batch biotransformations have been applied for preparative purposes

Published in AMB Express

ISSN: 2191-0855 (Online)
Publisher: SpringerOpen
Country of publisher: United Kingdom
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Microbiology
Website: http://www.amb-express.com/

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Abstract

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