Prevalence and Molecular Characterization of <i>Cryptosporidium</i> spp., <i>Giardia duodenalis</i>, and <i>Enterocytozoon bieneusi</i> in Cattle in Heilongjiang Province, Northeast China
Jun-Feng Gao,
Lu Zhou,
Ai-Hui Zhang,
Mei-Ru Hou,
Xue-Wei Liu,
Xin-Hui Zhang,
Jia-Wen Wang,
Xue Wang,
Xue Bai,
Chen-Long Jiao,
Yan Yang,
Zhuo Lan,
Hong-Yu Qiu,
Chun-Ren Wang
Affiliations
Jun-Feng Gao
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Lu Zhou
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Ai-Hui Zhang
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Mei-Ru Hou
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Xue-Wei Liu
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Xin-Hui Zhang
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Jia-Wen Wang
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Xue Wang
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Xue Bai
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Chen-Long Jiao
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Yan Yang
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Zhuo Lan
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Hong-Yu Qiu
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Chun-Ren Wang
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affair, Key Laboratory of Prevention and Control of Zoonotic Diseases of Daqing, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Crytosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi are important diarrheal pathogens with a global distribution that threatens the health of humans and animals. Despite cattle being potential transmission hosts of these protozoans, the associated risks to public health have been neglected. In the present study, a total of 1155 cattle fecal samples were collected from 13 administrative regions of Heilongjiang Province. The prevalence of Cryptosporidium spp., G. duodenalis, and E. bieneusi were 5.5% (64/1155; 95% CI: 4.2–6.9), 3.8% (44/1155; 95% CI: 2.7–4.9), and 6.5% (75/1155; 95% CI: 5.1–7.9), respectively. Among these positive fecal samples, five Cryptosporidium species (C. andersoni, C. bovis, C. ryanae, C. parvum, and C. occultus), two G. duodenalis assemblages (E and A), and eight E. bieneusi genotypes (BEB4, BEB6, BEB8, J, I, CHS7, CHS8, and COS-I) were identified. Phylogenetic analysis showed that all eight genotypes of E. bieneusi identified in the present study belonged to group 2. It is worth noting that some species/genotypes of these intestinal protozoans are zoonotic, suggesting a risk of zoonotic disease transmission in endemic areas. The findings expanded our understanding of the genetic composition and zoonotic potential of Cryptosporidium spp., G. duodenalis, and E. bieneusi in cattle in Heilongjiang Province.
