Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats

Angelina Schindele; Fabienne Gehrke; Carla Schmidt; Sarah Röhrig; Annika Dorn; Holger Puchta

doi:10.1038/s41467-022-29130-w

Nature Communications (Mar 2022)

Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats

Angelina Schindele,
Fabienne Gehrke,
Carla Schmidt,
Sarah Röhrig,
Annika Dorn,
Holger Puchta

Affiliations

Angelina Schindele: Botanical Institute – Molecular Biology and Biochemistry of Plants, Karlsruhe Institute of Technology
Fabienne Gehrke: Botanical Institute – Molecular Biology and Biochemistry of Plants, Karlsruhe Institute of Technology
Carla Schmidt: Botanical Institute – Molecular Biology and Biochemistry of Plants, Karlsruhe Institute of Technology
Sarah Röhrig: Botanical Institute – Molecular Biology and Biochemistry of Plants, Karlsruhe Institute of Technology
Annika Dorn: Botanical Institute – Molecular Biology and Biochemistry of Plants, Karlsruhe Institute of Technology
Holger Puchta: Botanical Institute – Molecular Biology and Biochemistry of Plants, Karlsruhe Institute of Technology

DOI: https://doi.org/10.1038/s41467-022-29130-w
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 10

Abstract

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How double strand breaks (DSBs) are repaired within the plant 45S rDNA repeats is unclear. Here, the authors show that Cas9-mediated DSBs in 45S rDNA are mainly repaired by cNHEJ and describe CRISPR-Kill as a tool for organ-specific cell elimination by targeting functional repetitive DNA in Arabidopsis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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