Analysis on epidemiological characteristics and PFGE typing of Campylobacter in diarrhea patients in Haidian District of Beijing from 2018 to 2020

Abstract

ObjectiveTo analyze and master the epidemiological characteristics and pulsed-field gel electrophoresis （PFGE） typing of Campylobacter in diarrhea patients in Haidian District of Beijing from 2018 to 2020.MethodsStool samples of 756 diarrhea patients were collected in 3 hospitals from 2018 to 2020. Campylobacter was isolated and identified， the isolated 25 Campylobacter strains were analyzed by PFGE for genetic typing.ResultsThe total detection rate of Campylobacter was 4.37% （33/756）， of which the detection rates of Campylobacter jejuni （C. jejuni） and Campylobacter coli （C. coli） were 3.04% （23/756） and 1.32% （10/756）， respectively. Epidemiological characteristics regarding the heterogeneities of Campylobacter showed no significant differences among different gender， age， and occupation. 48.48% （16/33） diarrhea patients experienced acute watery diarrhea， and they usually have a fever. The peak of detection rate sustained from May to July. Meat and meat products were recognized as a primary source of food-related transmission of Campylobacter to human. 16 and 7 patterns were detected through PFGE in C. jejuni strains and C. coli strains， respectively.ConclutionCampylobacter contamination deserved more attention. This study fills the data gap of Campylobacter infection in diarrhea patients in Haidian District of Beijing， and provides references for the prevention and control of foodborne diseases in Haidian District.

Keywords

• diarrhea patients
• campylobacter
• detection rate
• epidemiological characteristics
• pfge typing