Curcumin Modulates the Level of IL-17 and IL-10 Cytokines in Two Models of Experimental Liver Injury in Male Rats

Abstract

Introduction: Curcumin, a natural antioxidant, has anti-inflammatory and protective effect on a large number of diseases like cancers and hepatic disorders in oxidative stress conditions by collecting free oxygen radicals and increasing intracellular glutathione. The aim of this study was to determine the effects of curcumin on the level of IL-17 and IL-10 cytokines in intrahepatic and extrahepatic liver injuries Methods: A total of 72 male Wistar rats were randomly divided into two (A, B) categories, each of which was divided into 4 groups. A: One group as a control-sham group received distilled water as an acetaminophen vehicle and the other three groups received acetaminophen (500mg/kg IP). The third group received curcumin, and the fourth group was administrated curcumin vehicle. B: one group underwent Bile Duct Ligation (BDL), and another group received curcumin by gavage for seven days. The third group received distilled water as a curcumin vehicle and the fourth group was considered the sham group. Animals were sacrificed 48 hours after administration of acetaminophen under anesthesia with ketamine + xylazine. After that, liver tissue samples were taken for laboratory tests. Cytokines were measured by ELISA method. Results: Levels of IL-17 and IL-10 in the liver tissue in groups A and BDL increased significantly, and in the Curcumin (CMN) group, decreased significantly in both in- and out-liver injury. Also, the body weight in the curcumin-treated groups showed a significant increase both in intrahepatic and extrahepatic injuries. Conclusion: Our data suggest that curcumin undermines inflammation and damage to the inside and outside of the liver, but these findings need to be further investigated.

Keywords

• bile duct ligation interleukin
• 10 interleukin
• 17 rat curcumin cholestasis