Correlation of Glycated Haemoglobin with Protein Carbonyl Content as Biomarkers of Oxidative Stress in Type 2 Diabetes Mellitus

Abstract

Introduction: Diabetes is chronic disease which is characterised by defect in insulin secretion and insulin action leading to hyperglycaemia. Aim: To detect protein carbonyl as oxidative stress marker in Type 2 Diabetes Mellitus (T2DM). Materials and Methods: The present cross-sectional study was conducted on 100 subjects (aged 40 to 70 years) out of which 50 were T2DM and 50 were normal individuals. Serum was tested for Fasting Blood Sugar (FBS) and Glycated Haemoglobin (HbA1c) and Protein Carbonyl Content (PCC). Serum PCC was measured by spectrophotometric DNPH (dinitrophenylhydrazone) method of Reznick AZ and Packer L. Results: The protein carbonyl content, Fasting blood sugar and HbA1c were observed as positively correlated in type 2 diabetes mellitus as the levels of PCC, FBS and HbA1c were raised in T2DM as compared to controls. Conclusion: Increased HbA1c may be associated with generation of oxygen free radicals in the form of protein carbonyls and decreased antioxidants in T2DM.

Keywords

• dinitrophenylhydrazone
• fasting blood sugar
• hyperglycaemia