Nature Communications (Feb 2023)
Dissecting the immune suppressive human prostate tumor microenvironment via integrated single-cell and spatial transcriptomic analyses
- Taghreed Hirz,
- Shenglin Mei,
- Hirak Sarkar,
- Youmna Kfoury,
- Shulin Wu,
- Bronte M. Verhoeven,
- Alexander O. Subtelny,
- Dimitar V. Zlatev,
- Matthew W. Wszolek,
- Keyan Salari,
- Evan Murray,
- Fei Chen,
- Evan Z. Macosko,
- Chin-Lee Wu,
- David T. Scadden,
- Douglas M. Dahl,
- Ninib Baryawno,
- Philip J. Saylor,
- Peter V. Kharchenko,
- David B. Sykes
Affiliations
- Taghreed Hirz
- Center for Regenerative Medicine, Massachusetts General Hospital
- Shenglin Mei
- Center for Regenerative Medicine, Massachusetts General Hospital
- Hirak Sarkar
- Department of Biomedical Informatics, Harvard Medical School
- Youmna Kfoury
- Center for Regenerative Medicine, Massachusetts General Hospital
- Shulin Wu
- Department of Pathology, Massachusetts General Hospital, Harvard Medical School
- Bronte M. Verhoeven
- Childhood Cancer Research Unit, Department of Women’s and Children’s Health, Karolinska Institutet
- Alexander O. Subtelny
- Department of Pathology, Massachusetts General Hospital, Harvard Medical School
- Dimitar V. Zlatev
- Department of Urology, Massachusetts General Hospital, Harvard Medical School
- Matthew W. Wszolek
- Department of Urology, Massachusetts General Hospital, Harvard Medical School
- Keyan Salari
- Department of Urology, Massachusetts General Hospital, Harvard Medical School
- Evan Murray
- Broad Institute of Harvard and MIT
- Fei Chen
- Broad Institute of Harvard and MIT
- Evan Z. Macosko
- Broad Institute of Harvard and MIT
- Chin-Lee Wu
- Department of Pathology, Massachusetts General Hospital, Harvard Medical School
- David T. Scadden
- Center for Regenerative Medicine, Massachusetts General Hospital
- Douglas M. Dahl
- Department of Urology, Massachusetts General Hospital, Harvard Medical School
- Ninib Baryawno
- Childhood Cancer Research Unit, Department of Women’s and Children’s Health, Karolinska Institutet
- Philip J. Saylor
- Massachusetts General Hospital Cancer Center, Harvard Medical School
- Peter V. Kharchenko
- Harvard Stem Cell Institute
- David B. Sykes
- Center for Regenerative Medicine, Massachusetts General Hospital
- DOI
- https://doi.org/10.1038/s41467-023-36325-2
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 20
Abstract
The immune suppressive tumour microenvironment drives recurrence and metastatic disease in prostate cancer. Here authors provide a detailed analysis of the microenvironment via single cell RNA sequencing and high-resolution spatial transcriptomics to identify tumour-dependent changes compared to healthy tissue.
