Study of molasses / vinasse waste ratio for single cell protein and total microorganisms

Abstract

Different molasses/ vinasse ratio were used as substrate to investigate single cell protein and total lipids production by five microorganisms: four yeasts strains: Candida lipolytica, Rhodotorula mucilaginosa, Saccharomyces cerevisiae, a yeast isolated from vinasse lake (denominated LLV98) and a bacterium strain, Corynebacterium glutamicum. The media utilized were: a) 50% molasses and 50% vinasse; b) 25% molasses and 75% vinasse and c) 75% molasses and 25% vinasse. The objective of this work was to study the growth of microorganisms and also evaluate protein and lipids content in the biomass obtained from these by-products. The highest single cell protein production was obtained by S. cerevisiae, 50.35%, followed by R. mucilaginosa, 41.96%. The lowest productions were obtained by C. glutamicum. The higher total lipids productions, more than 26%, were founded in molasses plus vinasse at 50%/50% by S. cerevisiae and C. glutamicum.

Keywords

• Vinasse
• Molasses
• Single cell protein
• Total lipids
• Microorganisms