Asian Fisheries Science (Jul 2007)

Isolation and Development of DNA Microsatellite Markers for the River Catfish (Mystus nemurus)

  • B.P. HOH,
  • S.S. SIRAJ,
  • S.G. TAN,
  • K. YUSOFF

DOI
https://doi.org/10.33997/j.afs.2007.20.1.004
Journal volume & issue
Vol. 20, no. 1

Abstract

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The microsatellite markers for Mystus nemurus were isolated using the 5’ anchored PCR procedure. A degenerate primer was designed for the construction of the genomic library. Fourteen clones were sequenced and revealed a total of 45 microsatellite repeats. From these, 20 specific primer pairs were designed and employed to characterize unrelated fishes from six populations of M. nemurus. Thirteen loci were found to be polymorphic. The allelic frequencies ranged from 0.007 to 0.800, while the levels of heterozygosity ranged from 0.0563 (MnBp5-1-115b) to 0.8714 (MnBp5-1-12a).