Electrospun Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate)/Olive Leaf Extract Fiber Mesh as Prospective Bio-Based Scaffold for Wound Healing
Jose Gustavo De la Ossa,
Serena Danti,
Jasmine Esposito Salsano,
Bahareh Azimi,
Veronika Tempesti,
Niccoletta Barbani,
Maria Digiacomo,
Marco Macchia,
Mohammed Jasim Uddin,
Caterina Cristallini,
Rossella Di Stefano,
Andrea Lazzeri
Affiliations
Jose Gustavo De la Ossa
Cardiovascular Research Laboratory, Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, 56126 Pisa, Italy
Serena Danti
Department of Civil and Industrial Engineering, University of Pisa, 56122 Pisa, Italy
Jasmine Esposito Salsano
Doctoral School in Life Sciences, University of Siena, 53100 Siena, Italy
Bahareh Azimi
Department of Civil and Industrial Engineering, University of Pisa, 56122 Pisa, Italy
Veronika Tempesti
Department of Civil and Industrial Engineering, University of Pisa, 56122 Pisa, Italy
Niccoletta Barbani
Department of Civil and Industrial Engineering, University of Pisa, 56122 Pisa, Italy
Maria Digiacomo
Department of Pharmacy, University of Pisa, 56126 Pisa, Italy
Marco Macchia
Department of Pharmacy, University of Pisa, 56126 Pisa, Italy
Mohammed Jasim Uddin
Photonics and Energy Research Laboratory, Department of Chemistry, University of Texas Rio Grande Valley, Edinburg, TX 78539, USA
Caterina Cristallini
Institute for Chemical and Physical Processes (IPCF) @ Pisa, CNR, 56126 Pisa, Italy
Rossella Di Stefano
Cardiovascular Research Laboratory, Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, 56126 Pisa, Italy
Andrea Lazzeri
Department of Civil and Industrial Engineering, University of Pisa, 56122 Pisa, Italy
Polyhydroxyalkanoates (PHAs) are a family of biopolyesters synthesized by various microorganisms. Due to their biocompatibility and biodegradation, PHAs have been proposed for biomedical applications, including tissue engineering scaffolds. Olive leaf extract (OLE) can be obtained from agri-food biowaste and is a source of polyphenols with remarkable antioxidant properties. This study aimed at incorporating OLE inside poly(hydroxybutyrate-co-hydroxyvalerate) (PHBHV) fibers via electrospinning to obtain bioactive bio-based blends that are useful in wound healing. PHBHV/OLE electrospun fibers with a size of 1.29 ± 0.34 µm were obtained. Fourier transform infrared chemical analysis showed a uniform surface distribution of hydrophilic -OH groups, confirming the presence of OLE in the electrospun fibers. The main OLE phenols were released from the fibers within 6 days. The biodegradation of the scaffolds in phosphate buffered saline was investigated, demonstrating an adequate stability in the presence of metalloproteinase 9 (MMP-9), an enzyme produced in chronic wounds. The scaffolds were preliminarily tested in vitro with HFFF2 fibroblasts and HaCaT keratinocytes, suggesting adequate cytocompatibility. PHBHV/OLE fiber meshes hold promising features for wound healing, including the treatment of ulcers, due to the long period of durability in an inflamed tissue environment and adequate cytocompatibility.
