Multi-Omics Analysis to Generate Hypotheses for Mild Health Problems in Monkeys
Fumie Hamano,
Suzumi M. Tokuoka,
Megumi Ishibashi,
Yasuto Yokoi,
Dieter M. Tourlousse,
Yoshihiro Kita,
Yuji Sekiguchi,
Hiroyuki Yasui,
Takao Shimizu,
Yoshiya Oda
Affiliations
Fumie Hamano
Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8654, Japan
Suzumi M. Tokuoka
Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8654, Japan
Megumi Ishibashi
Thermo Fisher Scientific K. K., Moriya-cho 3-9, Kanagawa-ku, Yokohama-shi 221-0022, Japan
Yasuto Yokoi
Mitsui Knowledge Industry Co., Ltd., Atago Green Hills MORI Tower, Atago 2-5-1, Minato-ku, Tokyo 105-6215, Japan
Dieter M. Tourlousse
Biomedical Research Institute, Advanced Industrial Science and Technology (AIST), Central 6, Higashi 1-1-1, Tsukuba 305-8566, Japan
Yoshihiro Kita
Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8654, Japan
Yuji Sekiguchi
Biomedical Research Institute, Advanced Industrial Science and Technology (AIST), Central 6, Higashi 1-1-1, Tsukuba 305-8566, Japan
Hiroyuki Yasui
Department of Analytical & Bioinorganic Chemistry, Division of Analytical and Physical Chemistry, Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan
Takao Shimizu
Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8654, Japan
Yoshiya Oda
Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8654, Japan
Certain symptoms associated with mild sickness and lethargy have not been categorized as definitive diseases. Confirming such symptoms in captive monkeys (Macaca fascicularis, known as cynomolgus monkeys) can be difficult; however, it is possible to observe and analyze their feces. In this study, we investigated the relationship between stool state and various omics data by considering objective and quantitative values of stool water content as a phenotype for analysis. By examining the food intake of the monkeys and assessing their stool, urine, and plasma, we attempted to obtain a comprehensive understanding of the health status of individual monkeys and correlate it with the stool condition. Our metabolomics data strongly suggested that many lipid-related metabolites were correlated with the stool water content. The lipidomic analysis revealed the involvement of saturated and oxidized fatty acids, metallomics revealed the contribution of selenium (a bio-essential trace element), and intestinal microbiota analysis revealed the association of several bacterial species with the stool water content. Based on our results, we hypothesize that the redox imbalance causes minor health problems. However, it is not possible to make a definite conclusion using multi-omics alone, and other hypotheses could be proposed.
