糖尿病合并心脏功能障碍患者细胞外小囊泡中n‐3 DHA 富集的三酰甘油减少

Abstract

Abstract Purpose Diabetic cardiomyopathy is the leading cause of death in diabetic patients, and the mechanism by which factors other than hyperglycemia contribute to the development of diabetic cardiomyopathy is unknown. Serum small extracellular vesicles (sEVs) carry bioactive proteins or nuclei, which enter into remote tissues and modulate cell functions. However, in diabetic conditions, the changes of lipids carried by sEVs has not been identified. Our study aims to explore the changes of lipids in sEVs in diabetic patients with cardiovascular disease, we hope to provide new ideas for understanding the role of lipid metabolism in the pathogenesis of diabetic cardiomyopathy. Methods SEVs samples derived from serum of health controls (Ctrl), diabetic patients without cardiovascular diseases (DM), and diabetic patients with cardiovascular diseases (DM‐CAD) were used for lipidomics analysis. Because AC16 cells are also treated with those sEVs to confirm the entrance of cells and effects on insulin sensitivity, a lipidomics analysis on cells was also performed. Results and Conclusions In this study, we found that docosahexaenoic acid (DHA)‐triacylglycerides of sEVs from serums of DM‐CAD patients decreased significantly, and those sEVs could enter into AC16 cells and diminish insulin sensitivity. In addition, DHA‐triacylglycerides were also decreased in cells treated with sEVs from DM‐CAD. Therefore, DHA‐triacylglycerides carried by sEVs may mediate intercellular signaling and be associated with the incidence of diabetic cardiovascular complications.

Keywords

• 心脏功能障碍
• DHA
• 细胞外囊泡
• 胰岛素抵抗
• 三酰甘油