Changes in Proteins in Saliva and Serum in Equine Gastric Ulcer Syndrome Using a Proteomic Approach
Alberto Muñoz-Prieto,
Maria Dolores Contreras-Aguilar,
Jose Joaquín Cerón,
Ignacio Ayala,
Maria Martin-Cuervo,
Juan Carlos Gonzalez-Sanchez,
Stine Jacobsen,
Josipa Kuleš,
Anđelo Beletić,
Ivana Rubić,
Vladimir Mrljak,
Fernando Tecles,
Sanni Hansen
Affiliations
Alberto Muñoz-Prieto
Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
Maria Dolores Contreras-Aguilar
Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain
Jose Joaquín Cerón
Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain
Ignacio Ayala
Department of Animal Medicine & Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain
Maria Martin-Cuervo
Medicine Animal, Faculty of Veterinary Medicine of Cáceres, University of Extremadura, Avenida de la Universidad S-N, 10002 Cáceres, Spain
Juan Carlos Gonzalez-Sanchez
BioQuant, Faculty of Biosciences, Heidelberg University, Im Neuenheimer Feld 267, 69117 Heidelberg, Germany
Stine Jacobsen
Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, 2630 Taastrup, Denmark
Josipa Kuleš
Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
Anđelo Beletić
Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
Ivana Rubić
Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
Vladimir Mrljak
Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
Fernando Tecles
Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain
Sanni Hansen
Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, 2630 Taastrup, Denmark
Changes in the salivary proteome in 12 horses with the two diseases included in equine gastric ulcer syndrome (EGUS), equine glandular gastric disease (EGGD) (n = 6) and equine squamous gastric disease (ESGD) (n = 6), were evaluated using a high-resolution LC-MS/MS analysis of TMT-labelled peptides and compared to 10 healthy control horses. Serum was also analysed for comparative purposes. The comparison between the horses with EGGD and controls showed significant changes in 10 salivary proteins, whereas 36 salivary proteins were differently abundant between ESGD and control groups. The most upregulated proteins in the case of EGGD were related to immune activation whereas, in horses with ESGD, the most significantly changed proteins were associated with squamous cell regulation and growth. Compared to serum, saliva showed a higher number of proteins with significant changes and a different pattern of changes. The proteins identified in our study, in addition to providing new information about the pathophysiological mechanisms in these diseases, could have the potential to be novel biomarkers for the diagnosis or monitoring of EGGD and ESGD.
