In-Situ Electrophoretic Mobility Determination by Particle Image Velocimetry for Efficient Microfluidic Enrichment of Bacteria

Richard Rietzel; Matthias Hügle; Gregory Dame; Ole Behrmann; Gerald A. Urban

doi:10.3390/proceedings1040535

Proceedings (Aug 2017)

In-Situ Electrophoretic Mobility Determination by Particle Image Velocimetry for Efficient Microfluidic Enrichment of Bacteria

Richard Rietzel,
Matthias Hügle,
Gregory Dame,
Ole Behrmann,
Gerald A. Urban

Affiliations

Richard Rietzel: IMTEK—Department of Microsystems Engineering, University of Freiburg, 79085 Freiburg, Germany
Matthias Hügle: IMTEK—Department of Microsystems Engineering, University of Freiburg, 79085 Freiburg, Germany
Gregory Dame: Institute for Microbiology and Virology, Brandenburg Medical School, 16816 Neuruppin, Germany
Ole Behrmann: IMTEK—Department of Microsystems Engineering, University of Freiburg, 79085 Freiburg, Germany
Gerald A. Urban: IMTEK—Department of Microsystems Engineering, University of Freiburg, 79085 Freiburg, Germany

DOI: https://doi.org/10.3390/proceedings1040535
Journal volume & issue: Vol. 1, no. 4
p. 535

Abstract

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We present a novel approach for the efficiency enhancement of microfluidic bacteria enrichment systems based on free-flow electrophoresis (FFE). FFE efficiency is highly dependent on the electrophoretic mobility μ of the bacteria. As μ varies strongly with the suspension medium, fast and accurate determination of μ is needed to achieve optimal enrichment performance from different suspension media. For the first time, μ is determined in-situ for multiple media during on-chip FFE by Particle Image Velocimetry (PIV) of fluorescent bacteria, obviating the need for separate measurement equipment or chemical staining of the bacteria.

Published in Proceedings

ISSN: 2504-3900 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: General Works
Website: http://www.mdpi.com/journal/proceedings

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