BIOLOGICAL PROPERTIES OF ENTEROCOCCI OF VARIOUS ORIGINS

Abstract

Aim. Study the prevalence and intensity of anti-hemoglobin and anti-lactoferrin activity in enterococci as representatives of symbiotic intestine microflora and causative agents of infection-inflammatory diseases in children. Materials and methods. 95 strains of enterococci were used in the study, that were isolated from intestines of children during examination for dysbiosis, and 34 clinical isolates. Strain identification was carried out by using multiplex PCR. Anti-lactoferrin and anti-hemoglobin activity were determined by O.V.Bukharin et al. (2005). Results. A higher level of prevalence and intensity of anti-lactoferrin, anti-hemoglobin activity, as well as a combination of those properties was established in clinical isolates of Enterococcus faecalis and E. faecium compared with strains, isolated from intestines of healthy individuals. Conclusion. The studied properties could be used for differentiation of pathogenic and non-pathogenic strains of enterococci and during selection of inhibitory preparations, suitable for therapy of enterococci infections.

Keywords

• enterococci
• clinical isolate
• intestine microflora
• anti-lactoferrin activity
• antihemoglobin activity