Selection and identification of a novel ssDNA aptamer targeting human skeletal muscle

Shuming Sun; Han Liu; Yan Hu; Yanpeng Wang; Mingri Zhao; Yijun Yuan; Yafei Han; Yingying Jing; Jin Cui; Xiaoxiang Ren; Xiao Chen; Jiacan Su

Bioactive Materials (Feb 2023)

Selection and identification of a novel ssDNA aptamer targeting human skeletal muscle

Shuming Sun,
Han Liu,
Yan Hu,
Yanpeng Wang,
Mingri Zhao,
Yijun Yuan,
Yafei Han,
Yingying Jing,
Jin Cui,
Xiaoxiang Ren,
Xiao Chen,
Jiacan Su

Affiliations

Shuming Sun: Molecular Biology Research Center, Center for Medical Genetics, Hunan Province Key Laboratory of Basic and Applied Hematology, School of Life Sciences, Central South University, Changsha, 410078, China
Han Liu: Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China
Yan Hu: Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China
Yanpeng Wang: Molecular Biology Research Center, Center for Medical Genetics, Hunan Province Key Laboratory of Basic and Applied Hematology, School of Life Sciences, Central South University, Changsha, 410078, China
Mingri Zhao: Center for Medical Genetics & Hunan Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan, 410013, China
Yijun Yuan: Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan, 410082, China
Yafei Han: Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China
Yingying Jing: Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China
Jin Cui: Department of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Medical University, Shanghai, 200433, China; Corresponding author.
Xiaoxiang Ren: Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China; Corresponding author.
Xiao Chen: Department of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Medical University, Shanghai, 200433, China; Corresponding author.
Jiacan Su: Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China; Department of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Medical University, Shanghai, 200433, China; Corresponding author. Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China.

Journal volume & issue: Vol. 20
pp. 166 – 178

Abstract

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Skeletal muscle disorders have posed great threats to health. Selective delivery of drugs and oligonucleotides to skeletal muscle is challenging. Aptamers can improve targeting efficacy. In this study, for the first time, the human skeletal muscle-specific ssDNA aptamers (HSM01, etc.) were selected and identified with Systematic Evolution of Ligands by Exponential Enrichment (SELEX). The HSM01 ssDNA aptamer preferentially interacted with human skeletal muscle cells in vitro. The in vivo study using tree shrews showed that the HSM01 ssDNA aptamer specifically targeted human skeletal muscle cells. Furthermore, the ability of HSM01 ssDNA aptamer to target skeletal muscle cells was not affected by the formation of a disulfide bond with nanoliposomes in vitro or in vivo, suggesting a potential new approach for targeted drug delivery to skeletal muscles via liposomes. Therefore, this newly identified ssDNA aptamer and nanoliposome modification could be used for the treatment of human skeletal muscle diseases.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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