Rationale and objective of the study: In the study of coordination of motor abilities, motor performance tests without examining the internal structure of the movement were used. The objective of this study was to examine the kinesthetic differentiation capacity level and to stimulate muscles during a test among basketball players in intermediate and specialist stages of training. Material and methods: Among ten basketball players from two equal groups at different level of advancement in sport, who play at diffe rent positions on the field, kinesthetic differentiation capacity was assessed by the „Basketball shooting with different balls” test. In order to assess the muscle involvement during the test, they were connected to the EMG amplifier. Results: More advanced basketball players obtained better results in the test, by an average of 7.4 point out of 60. Tested muscle bioelectric tension was lower by 21.8% in advanced players and the difference in the non-dominant hand was as high as 34.6%. Conclusions: The research indicates that advanced players obtain better level of kinesthetic differentiation and their average muscle bioelectric tension during throws is lower compared to beginner players. The difference in bio-electrical muscle tension in the time of throwing different balls was smaller in basketball players who are more advanced in training, and in both groups in the dominant hand. Mean values of EMG signal in a selected time interval show greater variability among the more advanced players.