Hippocampal Unicellular Recordings and Hippocampal-dependent Innate Behaviors in an Adolescent Mouse Model of Alzheimer’s disease
Siddhartha Mondragón-Rodríguez,
Benito Ordaz,
Erika Orta-Salazar,
Sofia Díaz-Cintra,
Fernando Peña-Ortega,
George Perry
Affiliations
Siddhartha Mondragón-Rodríguez
National Council for Science and Technology (CONACYT), México, MéxicoDevelopmental Neurobiology and Neurophysiology, Institute of Neurobiology, National Autonomous University of México (UNAM), Querétaro, México
Benito Ordaz
Developmental Neurobiology and Neurophysiology, Institute of Neurobiology, National Autonomous University of México (UNAM), Querétaro, México
Erika Orta-Salazar
Developmental Neurobiology and Neurophysiology, Institute of Neurobiology, National Autonomous University of México (UNAM), Querétaro, México
Sofia Díaz-Cintra
Developmental Neurobiology and Neurophysiology, Institute of Neurobiology, National Autonomous University of México (UNAM), Querétaro, México
Fernando Peña-Ortega
Developmental Neurobiology and Neurophysiology, Institute of Neurobiology, National Autonomous University of México (UNAM), Querétaro, México
George Perry
Neuroscience Institute and Department of Biology, College of Sciences, University of Texas at San Antonio (UTSA), San Antonio, Texas
Transgenic mice have been used to make valuable contributions to the field of neuroscience and model neurological diseases. The simultaneous functional analysis of hippocampal cell activity combined with hippocampal dependent innate task evaluations provides a reliable experimental approach to detect fine changes during early phases of neurodegeneration. To this aim, we used a merge of patch-clamp with two hippocampal innate behavior tasks. With this experimental approach, whole-cell recordings of CA1 pyramidal cells, combined with hippocampal-dependent innate behaviors, have been crucial for evaluating the early mechanism of neurodegeneration and its consequences. Here, we present our protocol for ex vivo whole-cell recordings of CA1 pyramidal cells and hippocampal dependent innate behaviors in an adolescent (p30) mice.
