Quantification of Ceftaroline in Human Plasma Using High-Performance Liquid Chromatography with Ultraviolet Detection: Application to Pharmacokinetic Studies
Ana Alarcia-Lacalle,
Helena Barrasa,
Javier Maynar,
Andrés Canut-Blasco,
Carmen Gómez-González,
María Ángeles Solinís,
Arantxazu Isla,
Alicia Rodríguez-Gascón
Affiliations
Ana Alarcia-Lacalle
Pharmacokinetic, Nanotechnology and Gene Therapy Group (Pharma Nano Gene), Centro de Investigación Lascaray Ikergunea, Faculty of Pharmacy, University of the Basque Country UPV/EHU, 01006 Vitoria-Gasteiz, Spain
Helena Barrasa
Instituto de Investigación Sanitaria Bioaraba, 01009 Vitoria-Gasteiz, Spain
Javier Maynar
Instituto de Investigación Sanitaria Bioaraba, 01009 Vitoria-Gasteiz, Spain
Andrés Canut-Blasco
Instituto de Investigación Sanitaria Bioaraba, 01009 Vitoria-Gasteiz, Spain
Carmen Gómez-González
Instituto de Investigación Sanitaria Bioaraba, 01009 Vitoria-Gasteiz, Spain
María Ángeles Solinís
Pharmacokinetic, Nanotechnology and Gene Therapy Group (Pharma Nano Gene), Centro de Investigación Lascaray Ikergunea, Faculty of Pharmacy, University of the Basque Country UPV/EHU, 01006 Vitoria-Gasteiz, Spain
Arantxazu Isla
Pharmacokinetic, Nanotechnology and Gene Therapy Group (Pharma Nano Gene), Centro de Investigación Lascaray Ikergunea, Faculty of Pharmacy, University of the Basque Country UPV/EHU, 01006 Vitoria-Gasteiz, Spain
Alicia Rodríguez-Gascón
Pharmacokinetic, Nanotechnology and Gene Therapy Group (Pharma Nano Gene), Centro de Investigación Lascaray Ikergunea, Faculty of Pharmacy, University of the Basque Country UPV/EHU, 01006 Vitoria-Gasteiz, Spain
This study was conducted to develop a rapid, simple and reproducible method for the quantification of ceftaroline in plasma samples by high-performance liquid chromatography with ultraviolet detection (HPLC-UV). Sample processing consisted of methanol precipitation and then, after centrifugation, the supernatant was injected into the HPLC system, working in isocratic mode. Ceftaroline was detected at 238 nm at a short acquisition time (less than 5 min). The calibration curve was linear over the concentration range from 0.25 to 40 µg/mL, and the method appeared to be selective, precise and accurate. Ceftaroline in plasma samples was stable at −80 °C for at least 3 months. The method was successfully applied to characterize the pharmacokinetic profile of ceftaroline in two critically ill patients and to evaluate whether the pharmacokinetic/pharmacodynamic (PK/PD) target was reached or not with the dose regimen administered.
