Single-molecule characterization of extrinsic transcription termination by Sen1 helicase

S. Wang; Z. Han; D. Libri; O. Porrua; T. R. Strick

doi:10.1038/s41467-019-09560-9

Nature Communications (Apr 2019)

Single-molecule characterization of extrinsic transcription termination by Sen1 helicase

S. Wang,
Z. Han,
D. Libri,
O. Porrua,
T. R. Strick

Affiliations

S. Wang: Molecular Motors and Machines group, Ecole normale supérieure, Institut de Biologie de l’Ecole normale supérieure (IBENS), CNRS, INSERM, PSL Research University
Z. Han: Metabolism and Function of RNA in the Nucleus, Institut Jacques Monod, CNRS, University Paris Diderot, Sorbonne Paris Cité
D. Libri: Metabolism and Function of RNA in the Nucleus, Institut Jacques Monod, CNRS, University Paris Diderot, Sorbonne Paris Cité
O. Porrua: Metabolism and Function of RNA in the Nucleus, Institut Jacques Monod, CNRS, University Paris Diderot, Sorbonne Paris Cité
T. R. Strick: Molecular Motors and Machines group, Ecole normale supérieure, Institut de Biologie de l’Ecole normale supérieure (IBENS), CNRS, INSERM, PSL Research University

DOI: https://doi.org/10.1038/s41467-019-09560-9
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 11

Abstract

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Yeast’s Sen1 helicase is involved in the suppression of antisense transcription from bidirectional eukaryotic promoters. Here authors develop and utilize a quantitative single-molecule assay reporting on the kinetics of extrinsic eukaryotic transcription termination by the Sen1 helicase and a reaction intermediate in which the Pol II transcription bubble appears half-rewound.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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