Sensitivity of field tests, serological and molecular techniques for Plum Pox Virus detection in various tissues

Abstract

Sensitivity of field tests (AgriStrip and Immunochromato), DAS-ELISA, two step RT-PCR and real-time RT-PCR for Plum pox virus (PPV) detection was tested in various tissues of apricot, peach, plum and damson plum trees infected with isolates belonging to PPV-D, PPV-M or PPV-Rec, the three strains present in Slovenia. Flowers of apricot and plum in full bloom proved to be a very good source for detection of PPV. PPV could be detected with all tested techniques in symptomatic parts of leaves in May and with one exception even in the beginning of August, but it was not detected in asymptomatic leaves using field tests, DAS-ELISA and partly also molecular techniques. PPV was detected only in some of the samples of asymptomatic parts of the leaves with symptoms and of stalks by field tests and DAS-ELISA. Infections were not detected in buds in August using field tests or DAS-ELISA. Field tests are useful for confirmation of the PPV infection in symptomatic leaves, but in tissues without symptoms DAS-ELISA should be combined or replaced by molecular techniques.

Keywords

• plum pox potyvirus
• elisa
• diagnosis
• infection
• prunus persica
• peaches