Co-expression of circRNA in oral leukoplakia and oral lichen planus

YANG Jingwen; SONG Yuhan; XU siming; GE Shuyun; ZHOU Haiwen

doi:10.12016/j.issn.2096-1456.2023.11.003

口腔疾病防治 (Nov 2023)

Co-expression of circRNA in oral leukoplakia and oral lichen planus

YANG Jingwen ,
SONG Yuhan ,
XU siming ,
GE Shuyun ,
ZHOU Haiwen

Affiliations

YANG Jingwen: Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University National center for Stomatoloy, National Clinical Research Center for oral diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology
SONG Yuhan: Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University National center for Stomatoloy, National Clinical Research Center for oral diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology
XU siming: Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University National center for Stomatoloy, National Clinical Research Center for oral diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology
GE Shuyun: Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University National center for Stomatoloy, National Clinical Research Center for oral diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology
ZHOU Haiwen: Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University National center for Stomatoloy, National Clinical Research Center for oral diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology

DOI: https://doi.org/10.12016/j.issn.2096-1456.2023.11.003
Journal volume & issue: Vol. 31, no. 11
pp. 774 – 785

Abstract

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Objective To find any differentially expressed circRNAs in oral leukoplakia (OLK) and oral lichen planus (OLP), to investigate the possible role of circRNAs in the pathogenesis of these two diseases. Methods This study obtained hospital ethical approval. High-throughput sequencing was used to detect differentially expressed circRNAs in OLK, OLP, oral squamous cell carcinoma and normal oral mucosal tissues. CircRNAs were verified by qRT-PCR, enzyme tolerance assays and Sanger sequencing. GO functional analysis and KEGG pathway analysis were performed to predict the functions of circRNAs in OLP. TargetScan and miRanda were applied to predict targeted miRNAs and mRNAs of circRNAs, and ceRNA networks were mapped. Results A total of 49 circRNAs were differentially expressed in OLK and OLP together, including 30 upregulated and 19 downregulated circRNAs. The five circRNAs confirmed with RT-qPCR, including circHLA-C, circRNF13, circTTN, circSEPN2 and circALDH3A2, were all abnormally expressed in OLK and OLP, among which circHLA-C was a key circRNA with trans splice sites, which was validated by expanding the sample size. ROC curve analysis showed that the area under the circHLA-C curve for predicting OLK was 0.955, and the area under the circHLA-C curve for predicting OLP was 0.988. GO functional analysis showed enrichment of many biological processes related to the immune process. The KEGG pathway with the highest enrichment score was "Natural killer cell mediated cytotoxicity". HLA-C was significantly enriched in these processes/pathways. CeRNA network analysis showed that circHLA-C interacted with a variety of miRNAs, such as hsa-miR-26a-5p, hsa-miR-129-5p, and hsa-miR-29a-3p. Conclusion Many circRNAs were differentially expressed in both OLK and OLP, circHLA-C being the most elevated. CircHLA-C is valuable for the early diagnosis of OLK and OLP and may serve as a potential biomarker for the diagnosis and prognosis of OLK and OLP.

Published in 口腔疾病防治

ISSN: 2096-1456 (Print)
Publisher: Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
Country of publisher: China
LCC subjects: Medicine
Website: http://www.kqjbfz.com/EN/2096-1456/home.shtml

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