<i>Lactobacillus plantarum</i> CCFM405 against Rotenone-Induced Parkinson’s Disease Mice via Regulating Gut Microbiota and Branched-Chain Amino Acids Biosynthesis

Chuanqi Chu; Leilei Yu; Yiwen Li; Hang Guo; Qixiao Zhai; Wei Chen; Fengwei Tian

doi:10.3390/nu15071737

Nutrients (Apr 2023)

<i>Lactobacillus plantarum</i> CCFM405 against Rotenone-Induced Parkinson’s Disease Mice via Regulating Gut Microbiota and Branched-Chain Amino Acids Biosynthesis

Chuanqi Chu,
Leilei Yu,
Yiwen Li,
Hang Guo,
Qixiao Zhai,
Wei Chen,
Fengwei Tian

Affiliations

Chuanqi Chu: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Leilei Yu: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Yiwen Li: Department of Food Science and Technology, The University of Georgia, Athens, GA 30602, USA
Hang Guo: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Qixiao Zhai: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Wei Chen: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Fengwei Tian: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China

DOI: https://doi.org/10.3390/nu15071737
Journal volume & issue: Vol. 15, no. 7
p. 1737

Abstract

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Recent studies have demonstrated that disturbances in the gut microbiota and microbiota -derived metabolites contribute to the pathogenesis of Parkinson’s disease (PD), suggesting that probiotic treatments that restore them may delay disease progression. This study aimed to examine the attenuating efficacy of L. plantarum CCFM405 and the potential mechanisms in mice with rotenone-induced PD. Our results indicate that L. plantarum CCFM405 ameliorated rotenone-induced motor deficits and constipation, decreased dopaminergic neuronal death, reduced intestinal inflammation and neuroinflammation, and raised dopamine levels, 5-HT, and associated metabolites in the striatal region of the brain in mice with PD. Sequencing of 16S rRNA from fecal microbiota revealed that L. plantarum CCFM405 normalized the gut bacterial composition in mice with PD, as evidenced by the increased relative abundance of the following genus, Bifidobacterium, Turicibacter, and Faecalibaculum, and decreased relative abundance of Alistipes, Bilophila, Akkermansia, and Escherichia-Shigella. The PICRUSt-predicted gut microbiota function revealed that L. plantarum CCFM405 enhanced the biosynthesis of amino acid pathways, particularly valine, leucine, and isoleucine (branched-chain amino acids, BCAAs). A non-metabolomic analysis of the serum and feces showed that L. plantarum CCFM405 markedly increased the levels of BCAAs. Pathway enrichment analysis based on the KEGG database further suggested that L. plantarum CCFM405 supplementation can promote BCAAs biosynthesis. Collectively, L. plantarum CCFM405 can help to prevent rotenone-induced PD by modulating the gut microbiota–metabolite axis. BCAAs may play a dominant role in L. plantarum CCFM405-associated neuroprotection in PD mice. This probiotic could be utilized as a potential food supplement in the management of PD.

Published in Nutrients

ISSN: 2072-6643 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.mdpi.com/journal/nutrients

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