Structural basis for CSPG4 as a receptor for TcdB and a therapeutic target in Clostridioides difficile infection

Peng Chen; Ji Zeng; Zheng Liu; Hatim Thaker; Siyu Wang; Songhai Tian; Jie Zhang; Liang Tao; Craig B. Gutierrez; Li Xing; Ralf Gerhard; Lan Huang; Min Dong; Rongsheng Jin

doi:10.1038/s41467-021-23878-3

Nature Communications (Jun 2021)

Structural basis for CSPG4 as a receptor for TcdB and a therapeutic target in Clostridioides difficile infection

Peng Chen,
Ji Zeng,
Zheng Liu,
Hatim Thaker,
Siyu Wang,
Songhai Tian,
Jie Zhang,
Liang Tao,
Craig B. Gutierrez,
Li Xing,
Ralf Gerhard,
Lan Huang,
Min Dong,
Rongsheng Jin

Affiliations

Peng Chen: Department of Physiology and Biophysics, University of California
Ji Zeng: Department of Urology, Boston Children’s Hospital, Harvard Medical School
Zheng Liu: Department of Physiology and Biophysics, University of California
Hatim Thaker: Department of Urology, Boston Children’s Hospital, Harvard Medical School
Siyu Wang: Department of Urology, Boston Children’s Hospital, Harvard Medical School
Songhai Tian: Department of Urology, Boston Children’s Hospital, Harvard Medical School
Jie Zhang: Department of Urology, Boston Children’s Hospital, Harvard Medical School
Liang Tao: Center for Infectious Disease Research, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University
Craig B. Gutierrez: Department of Physiology and Biophysics, University of California
Li Xing: UC Irvine Materials Research Institute (IMRI), University of California
Ralf Gerhard: Institute of Toxicology, Hannover Medical School
Lan Huang: Department of Physiology and Biophysics, University of California
Min Dong: Department of Urology, Boston Children’s Hospital, Harvard Medical School
Rongsheng Jin: Department of Physiology and Biophysics, University of California

DOI: https://doi.org/10.1038/s41467-021-23878-3
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 13

Abstract

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Chondroitin sulfate proteoglycan 4 (CSPG4) is a potential receptor for C. difficile toxin B (TcdB) during C. difficile infections (CDIs). Here, the cryo-EM structure of a TcdB–CSPG4 complex and CDI mouse models offer insights into CSPG4 role in CDIs and suggest a therapeutic strategy targeting TcdB.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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