Effects of death delay time on brain tissue staining based on synchronous X-ray multimodal imaging

Abstract

BackgroundHuman brain tissue is usually obtained by donating, and post-mortem delay (PMD) is one of the important factors affecting Golgi staining in human brain. However, currently there are relatively few studies on how PMD affects Golgi staining in human brain tissue.PurposeThis study aims to explore the changes in the morphology and structure of pyramidal neurons in the cerebral cortex of mice with PMD.MethodThe changes of human brain PMD were simulated by using mouse brain tissues placed in vitro for different times, and light microscope imaging data were obtained by Golgi staining and Nissl staining. At the same time, the synchrotron X-ray micro-CT imaging technology was used to realize neurons three-dimensional imaging visualization. The Sholl analysis was employed to study the Golgi-stained pyramidal neurons in the cerebral cortex.ResultsAnalysis results show that the completeness and complexity emerge a significant decreasing trend with the increase of PMD, and the statistical results of dendritic spines also show the same decrease trend. The three-dimensional neuron visualization results reconstructed by synchrotron radiation X-ray micro-CT technology confirm the above analysis results.ConclusionsThis study provides a research foundation for the establishment of Golgi staining method of human brain tissue and synchrotron radiation X-ray imaging.

Keywords

• cortical pyramidal neurons
• neuron structure
• golgi staining
• synchrotron x-ray micro-ct technology