We present the first record for Theraphosa apophysis (Tinter, 1991) for Brazil. A male of T. apophysis was collected in São Gabriel da Cachoeira, Amazonas state, Brazil. This is the third species of Theraphosa in Brazil along with T. blondi (Latreille, 1804) and T. stirmi Rudloff & Weinmann, 2010.
