Insulin like growth factor 2 (Igf2) and its receptor gene (Igf2r) showed opposite expression in diploid parthenogenetic embryos in Capra hircus

Abstract

The present research work was proposed to compare the expression of development related genes (Igf2 and Igf2r) among diploid parthenogenetic (DIP), female sexed in vivo and female sexed in vitro produced embryonic cell colony. The good quality caprine oocytes were matured in presence of cytochalasin B (CCB) and activated by ethanol (7%) for 5 min followed by incubation with 2 mM 6-dimethyl amino purine (DMAP) for 4 h for DIP embryos production and embryo development was recorded. We have explored comparative expression profile of paternally (Igf2r) and maternally (Igf2) imprinted genes among DIP, female sexed IVF and female sexed in vivo derived embryos. Embryonic cell colony were developed from 8–16 and morula stage DIP, IVF and in vivo derived embryos and these colonies were used for studying the paternal and maternal imprinted genes. Overall, we observed higher expression of maternally expressed genes (Igf2r) in DIP compared to in vivo and IVF derived embryonic cell colony. The expression of paternal genes (Igf2) was low in DIP compared to in vivo and IVF derived embryonic cell colony.

Keywords

• Cytochalasin B
• Diploid embryos
• Igf2r
• Igf2
• Parthenogenesis