Arabian Journal of Chemistry (Aug 2021)

A fast and efficient preparative method for separation and purification of main bioactive xanthones from the waste of Garcinia mangostana L. by high-speed countercurrent chromatography

  • Ricardo Felipe Alexandre de Mello,
  • Wandson B. de Souza Pinheiro,
  • Jaisielle Kelem F. Benjamim,
  • Francilia Campos de Siqueira,
  • Renan Campos Chisté,
  • Alberdan Silva Santos

Journal volume & issue
Vol. 14, no. 8
p. 103252

Abstract

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In this work, we present a preparative elution method by high-speed countercurrent chromatography (Prep-HSCCC) for the separation and isolation of the main bioactive xanthones present in the alcoholic extract of Garcinia mangostana L. agro-industrial waste. The method proved to be quite efficient, operating with a two-phase solvent system consisting of Methanol/water/Ethanol/Hexane/methyl tert-butyl ether (6:3:1:6:4v/v), tail-head elution mode, with mobile phase flow of 5 mL/min and rotation speed of 800 rpm. The method demonstrated repeatability (RSD = 3.8–6.5%) and allowed the isolation of α-mangostin and γ-mangostin xanthones, both with purities above 93%, in a single step, performed in a time of 35 min. Isolated substances were identified by HPLC-PDA-MS and 1H and 13C NMR spectroscopy. Both xanthones were efficient scavengers of DPPH·· and ABTS·+ radicals in a concentration-dependent manner with EC50 at 8.43 and 3.31 µg/mL, respectively, for γ-mangostin, and 71.10 and 29.33 µg/mL, respectively, for α-mangostin.

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