Generation and characterization of the human induced pluripotent stem cell (hiPSC) line NCUFi001-A from a patient carrying KCNQ1 G314S mutation

Luca Lavra; Fiorenza Magi; Alessandra Ulivieri; Alessandra Morgante; Marianna Paulis; Luca Sala; Matteo Pedrazzini; Patrizio Polisca; Marcella Rocchetti; Leonardo Calò; Salvatore Sciacchitano; Leila B. Salehi

Stem Cell Research (Jul 2021)

Generation and characterization of the human induced pluripotent stem cell (hiPSC) line NCUFi001-A from a patient carrying KCNQ1 G314S mutation

Luca Lavra,
Fiorenza Magi,
Alessandra Ulivieri,
Alessandra Morgante,
Marianna Paulis,
Luca Sala,
Matteo Pedrazzini,
Patrizio Polisca,
Marcella Rocchetti,
Leonardo Calò,
Salvatore Sciacchitano,
Leila B. Salehi

Affiliations

Luca Lavra: Laboratory of Biomedical Research, Niccolò Cusano University Foundation, Via Don Carlo Gnocchi 3, 00166 Rome, Italy; Corresponding author.
Fiorenza Magi: Laboratory of Biomedical Research, Niccolò Cusano University Foundation, Via Don Carlo Gnocchi 3, 00166 Rome, Italy
Alessandra Ulivieri: Laboratory of Biomedical Research, Niccolò Cusano University Foundation, Via Don Carlo Gnocchi 3, 00166 Rome, Italy
Alessandra Morgante: Laboratory of Biomedical Research, Niccolò Cusano University Foundation, Via Don Carlo Gnocchi 3, 00166 Rome, Italy
Marianna Paulis: Humanitas Clinical and Research Center-IRCCS, Rozzano, 20089 Milan, Italy; Institute of Genetic and Biomedical Research (IRGB)-UOS of Milan, National Research Council (CNR), 20138 Milan, Italy
Luca Sala: Center for Cardiac Arrhythmias of Genetic Origin and Laboratory of Cardiovascular Genetics, Istituto Auxologico Italiano IRCCS, Via Zucchi 18, Cusano Milanino, MI, Italy
Matteo Pedrazzini: Center for Cardiac Arrhythmias of Genetic Origin and Laboratory of Cardiovascular Genetics, Istituto Auxologico Italiano IRCCS, Via Zucchi 18, Cusano Milanino, MI, Italy
Patrizio Polisca: Laboratory of Biomedical Research, Niccolò Cusano University Foundation, Via Don Carlo Gnocchi 3, 00166 Rome, Italy; Department of Biomedicine and Prevention, University of Rome Tor Vergata, Viale Montpellier 1, 00133 Rome, Italy
Marcella Rocchetti: Department of Biotechnology and Biosciences, Università degli Studi di Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy
Leonardo Calò: Division of Cardiology, Policlinico Casilino, Via Casilina 1049, 00169 Rome, Italy
Salvatore Sciacchitano: Laboratory of Biomedical Research, Niccolò Cusano University Foundation, Via Don Carlo Gnocchi 3, 00166 Rome, Italy; Department of Clinical and Molecular Medicine, Sapienza University, Via di Grottarossa 1035/1039, 00189 Rome, Italy
Leila B. Salehi: Laboratory of Biomedical Research, Niccolò Cusano University Foundation, Via Don Carlo Gnocchi 3, 00166 Rome, Italy; Medical Genetics Unit, Tor Vergata University Hospital, PTV, Viale Montpellier 1, 00133 Rome, Italy

Journal volume & issue: Vol. 54
p. 102418

Abstract

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In this study we describe the generation and characterization of an human induced pluripotent stem cell (hiPSC) line from a long QT syndrome type 1 (LQT1) patient carrying the KCNQ1 c.940 G > A (p.Gly314Ser) mutation. This patient-specific iPSC line has been obtained by using non-integrational Sendai reprogramming method, expresses pluripotency markers and has the capacity to differentiate into the three germ layers and into spontaneously beating cardiomyocytes (iPSC-CMs).

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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