Frontiers in Molecular Neuroscience (Dec 2024)
Characterization of 3,3′-iminodipropionitrile (IDPN) damaged utricle transcriptome in the adult mouse utricle
- Mengyao Tian,
- Mengyao Tian,
- Jingyuan Huang,
- Jingyuan Huang,
- Hairong Xiao,
- Hairong Xiao,
- Pei Jiang,
- Pei Jiang,
- Xiangyu Ma,
- Xiangyu Ma,
- Yanqin Lin,
- Yanqin Lin,
- Xujun Tang,
- Xujun Tang,
- Yintao Wang,
- Yintao Wang,
- Mingchen Dai,
- Mingchen Dai,
- Wei Tong,
- Wei Tong,
- Zixuan Ye,
- Zixuan Ye,
- Xia Sheng,
- Xia Sheng,
- Renjie Chai,
- Renjie Chai,
- Renjie Chai,
- Renjie Chai,
- Renjie Chai,
- Renjie Chai,
- Shasha Zhang,
- Shasha Zhang
Affiliations
- Mengyao Tian
- State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China
- Mengyao Tian
- Southeast University, Shenzhen Research Institute, Shenzhen, China
- Jingyuan Huang
- State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China
- Jingyuan Huang
- Southeast University, Shenzhen Research Institute, Shenzhen, China
- Hairong Xiao
- State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China
- Hairong Xiao
- Southeast University, Shenzhen Research Institute, Shenzhen, China
- Pei Jiang
- State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China
- Pei Jiang
- Southeast University, Shenzhen Research Institute, Shenzhen, China
- Xiangyu Ma
- State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China
- Xiangyu Ma
- Southeast University, Shenzhen Research Institute, Shenzhen, China
- Yanqin Lin
- State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China
- Yanqin Lin
- Southeast University, Shenzhen Research Institute, Shenzhen, China
- Xujun Tang
- State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China
- Xujun Tang
- Southeast University, Shenzhen Research Institute, Shenzhen, China
- Yintao Wang
- State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China
- Yintao Wang
- Southeast University, Shenzhen Research Institute, Shenzhen, China
- Mingchen Dai
- State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China
- Mingchen Dai
- Southeast University, Shenzhen Research Institute, Shenzhen, China
- Wei Tong
- State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China
- Wei Tong
- Southeast University, Shenzhen Research Institute, Shenzhen, China
- Zixuan Ye
- State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China
- Zixuan Ye
- Southeast University, Shenzhen Research Institute, Shenzhen, China
- Xia Sheng
- School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Xia Sheng
- Department of Environmental Health, School of Environmental Science and Engineering, Hainan University, Haikou, China
- Renjie Chai
- State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China
- Renjie Chai
- Southeast University, Shenzhen Research Institute, Shenzhen, China
- Renjie Chai
- Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, China
- Renjie Chai
- Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, China
- Renjie Chai
- Institute for Stem Cell and Regeneration, Chinese Academy of Science, Beijing, China
- Renjie Chai
- Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing, China
- Shasha Zhang
- State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China
- Shasha Zhang
- Southeast University, Shenzhen Research Institute, Shenzhen, China
- DOI
- https://doi.org/10.3389/fnmol.2024.1487364
- Journal volume & issue
-
Vol. 17
Abstract
Utricle is an important vestibular sensory organ for maintaining balance. 3,3′-iminodipropionitrile (IDPN), a prototype nitrile toxin, has been reported to be neurotoxic and vestibulotoxic, and can be used to establish an in vivo damage model of vestibular dysfunction. However, the mechanism of utricular HCs damage caused by IDPN is unclear. Here, we first studied mice balance behavior and HCs damage in IDPN utricle damage model, and found that IDPN injection in vivo can cause vestibular dysfunction and HCs damage, which is more pronounced than neomycin damage model. Then we used RNA-seq to characterize the transcriptome of IDPN damaged utricle in detail to identify genes and pathways that play roles in this process. We found 1,165 upregulated genes and 1,043 downregulated genes in IDPN damaged utricles, and identified that NF-κB pathway and TNF pathway may play important roles in IDPN damage model. Our study provides details of transcriptome of IDPN utricle damage model for further study of vestibular dysfunction.
Keywords
