The Effect of Olive Leaf Smoke on the Blood Factors and Tissues in Rats

Abstract

Background and Objectives: This study aims to assess the effect of olive leaf smoke inhalation on the blood Biochemical factors, activity of liver enzymes, and the changes in liver, lung and kidney tissues in male Wistar rats. Methods: In this experimental study, 21 male Wistar rats were put in three groups of 7, including control, olive smoke for one hour per day, and olive smoke for two hours per day. The levels of glucose, triglyceride, cholesterol, urea, creatinine, low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), and the activity of liver enzymes including alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (GGT) were measured. Histological changes in liver, kidney, and lung tissues were also investigated. Finally, the data were analyzed statistically by one-way analysis of variance. The significant level was set at P0.05). However, it significantly increased the activity of AST and GGT enzymes compared to two other groups (P<0.001). Treatment with olive leaf smoke for one hour did not cause significant changes in these factors. Conclusion: Treatments with olive leaf smoke for 1 or 2 hours do not cause significant changes in blood biochemical factors. However, the 2-hour treatment can affect the activity of liver enzymes AST, ALP and GGT. The olive leaf smoke has pathological effects on liver, lung and kidney tissues.

Keywords

• kidney
• liver
• lung
• olea europea
• smoke