Enhancing Potato Virus X Diagnosis: Chelex ™ 100 Resin RNA Extraction Method Evaluation

Abstract

Potato Virus X (PVX) is a prevalent pathogen affecting potato crops, leading to substantial yield losses. This study investigates the efficacy of Chelex™ 100 resin RNA extraction method for PVX detection, through RT-qPCR analysis. We evaluate RNA yield, sensitivity, and detection limits of this method compared with Phenol-chloroform RNA extraction protocol. Our results demonstrate that Chelex™ 100 resin extraction yields superior RNA concentrations and exhibits greater sensitivity compared to ELISA immunoassay diagnostic test. RT-qPCR successfully detects PVX RNA in dilutions up to 1:100 using Chelex™ 100 resin extraction. These findings highlight the potential of Chelex™ 100 resin extraction coupled with RT-qPCR as a rapid and reliable method for PVX detection.

Keywords

• PVX
• Chelex
• Molecular Diagnostics
• Plant Virus