Purpose: The effect of physical exercise on lipid peroxidation was investigated. Method: 27 healthy young adult male subjects were included in this study. Urine samples were collected before and after exercises. Urinary malondialdehyde and creatinine levels (Cr) were measured. Results: Urinary malondialdehyde levels were increased by exercise. While pre-exercise malondialdehyde levels were 5.02±1.26 nmol/mg Cr, post-exercise levels were 6.13±1.84 nmol/mg Cr (p<0.05). Conclusion: These findings indicated that physical exercise induced lipid peroxidation.