Generation and characterisation of monoclonal antibodies specific to Plasmodium falciparum enolase

Abstract

Background & objectives: Glycolysis is the sole source of energy for the intraerythrocytic stages ofPlasmodium falciparum, making glycolytic enzymes putative therapeutic targets. Enolase, a singlecopy gene in P. falciparum is one such enzyme whose activity is elevated ~10–15 fold in infectedRBC’s. It holds the possibility of having multiple biological functions in the parasite and hence canbe a suitable candidate for diagnostic and chemotherapeutic purposes.Methods: We have aimed at generating parasite-specific reagents in the form of monoclonalantibodies. We have raised monoclonal antibodies against the recombinant P. falciparum enolase.Results: Two IgG monoclonals were obtained with 1:1000 titre and specific for P. falciparum enolase.Apicomplexan parasites including P. falciparum enolase has a plant like pentapeptide sequence(104EWGWS108) which is uniquely different from the host counterpart. A peptide spanning thispentapeptide region (ELDGSKNEWGWSKSK) coupled to BSA was used to raise parasite-specificantibody. Four monoclonals were obtained with 1:1000 titre and of IgM isotype.Interpretation & conclusion: All the monoclonals are specific for P. falciparum enolase and one ofthem display reactivity against native P. falciparum enolase signifying this pentapeptide to be surfaceexposed and immunogenic

Keywords

• ELISA
• enolase
• monoclonal antibodies
• Plasmodium falciparum