Endosperm protein polymorphism of common and tartary buckwheat

Abstract

The endosperm protein polymorphism of seven accessions of common buckwheat (Fagopyrum esculentum Moench) and two accessions of tartary buckwheat (Fagopyrum tataricum Gaertn.) were studied by SDS-PAGE electrophoresis. The position and the intensity of the main four electrophoretic protein bands (marked as “a”, “b”, “c” and “d”) of cultivar Siva (common buckwheat) were estimated and the polymorphism was studied using seeds from open pollinated heterozygous plants. Two high significant correlations (correlation coefficient 0.559 for “a” and “c” bands, and 0.264 for “a” and “d” bands) were established among the intensities of electrophoretic protein bands of common buckwheat, while other four possible relations were not significant. The results indicate significantly higher protein polymorphism of endosperm within individual accessions than among accessions of common buckwheat. In contrary to common buckwheat, there was in material studied found no variability in endosperm protein bands between or within two accessions of tartary buckwheat.

Keywords

• Fagopyrum esculentum
• Fagopyrum tataricum
• SDS-PAGE electrophoresis
• endosperm proteins
• polymorphism