Label-free automated three-dimensional imaging of whole organs by microtomy-assisted photoacoustic microscopy

Terence T. W. Wong; Ruiying Zhang; Chi Zhang; Hsun-Chia Hsu; Konstantin I. Maslov; Lidai Wang; Junhui Shi; Ruimin Chen; K. Kirk Shung; Qifa Zhou; Lihong V. Wang

doi:10.1038/s41467-017-01649-3

Nature Communications (Nov 2017)

Label-free automated three-dimensional imaging of whole organs by microtomy-assisted photoacoustic microscopy

Terence T. W. Wong,
Ruiying Zhang,
Chi Zhang,
Hsun-Chia Hsu,
Konstantin I. Maslov,
Lidai Wang,
Junhui Shi,
Ruimin Chen,
K. Kirk Shung,
Qifa Zhou,
Lihong V. Wang

Affiliations

Terence T. W. Wong: Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology
Ruiying Zhang: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis
Chi Zhang: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis
Hsun-Chia Hsu: Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology
Konstantin I. Maslov: Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology
Lidai Wang: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis
Junhui Shi: Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology
Ruimin Chen: NIH Resource Center for Medical Ultrasonic Transducer Technology, Department of Biomedical Engineering, University of Southern California
K. Kirk Shung: NIH Resource Center for Medical Ultrasonic Transducer Technology, Department of Biomedical Engineering, University of Southern California
Qifa Zhou: NIH Resource Center for Medical Ultrasonic Transducer Technology, Department of Biomedical Engineering, University of Southern California
Lihong V. Wang: Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology

DOI: https://doi.org/10.1038/s41467-017-01649-3
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 8

Abstract

Read online

The state-of-the-art three-dimensional biomedical imaging often requires specific tissue preparation that may alter the physical properties of the specimen causing loss of information. Here Wong et al. develop a microtomy-assisted photoacoustic microscopy that allows imaging of biological samples without labelling agents and with reduced sectioning.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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