Advance technologies for DNA-protein interactions and future research prospect

Abstract

DNA-protein interactions (DPIs) are essential for genome functioning, with billions of years of evolution shaping specific patterns of protein-DNA interactions to regulate gene networks in response to various stimuli. Over the years, scientists have developed numerous techniques to study these interactions. This review provides a historical overview of these methods, highlighting their advantages and disadvantages and offering the examples of recent applications. Our aim is to help researchers select the most appropriate technique on the basis of their working goals and capabilities. For the experimental design of DPIs assays, several kinds of techniques are relatively quicker or/and simpler, the precision and accuracy of these methods must be carefully considered to verify the DNA-protein interaction. The review also discusses the recent advances in the computational approaches for predicting DNA-protein bindings and diverse reporting systems.

Keywords

• DNA-protein interaction
• Electrophoretic mobility shift assay
• Enzyme-linked immunosorbent assay
• Footprinting
• CUT&RUN
• Chromatin immunoprecipitation