Investigating the effectiveness of iron nanoparticles synthesized by green synthesis method in chemoradiotherapy of colon cancer
Farshad Seyed Nejad,
Mostafa Alizade-Harakiyan,
Mehdi Haghi,
Rokhsareh Ebrahimi,
Mohammad Mahdi Zangeneh,
Alireza Farajollahi,
Roghayeh Fathi,
Reza Mohammadi,
Samira Samadi Miandoab,
Mohammad Heydarnezhad Asl,
Parina Asgharian,
Baharak Divband,
Amin Ahmadi
Affiliations
Farshad Seyed Nejad
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Radiation Oncology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Mostafa Alizade-Harakiyan
Department of Radiation Oncology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Medical Physics Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Corresponding author. Department of Radiation Oncology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Mehdi Haghi
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran; Corresponding author.
Rokhsareh Ebrahimi
Medicinal Chemistry Department, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Mohammad Mahdi Zangeneh
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran; Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran
Alireza Farajollahi
Department of Radiation Oncology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Medical Physics Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Medical Radiation Science Research Team, Tabriz University of Medical Sciences, Tabriz, Iran
Roghayeh Fathi
Polymer Research Laboratory, Department of Organic and Biochemistry, University of Tabriz, Tabriz, Iran
Reza Mohammadi
Polymer Research Laboratory, Department of Organic and Biochemistry, University of Tabriz, Tabriz, Iran
Samira Samadi Miandoab
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Mohammad Heydarnezhad Asl
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
Parina Asgharian
Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Baharak Divband
Department of Inorganic Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran
Amin Ahmadi
Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran
Current methods of colon cancer treatment, especially chemotherapy, require new treatment methods due to adverse side effects. One important area of interest in recent years is the use of nanoparticles as drug delivery vehicles since several studies have revealed that they can improve the target specificity of the treatment thus lowering the dosage of the drugs while preserving the effectiveness of the treatment thus reducing the side effects. The use of traditional medicine has also been a favorite topic of interest in recent years in medical research, especially cancer research. In this research work, the green synthesis of Fe nanoparticles was carried out using Mentha spicata extract and the synthesized nanoparticles were identified using FT-IR, XRD, FE-SEM and EDS techniques. Then the effect of Mentha spicata, Fe nanoparticles, and Mentha spicata -loaded Fe nanoparticles on LS174t colon cancer cells, and our result concluded that all three, especially Mentha spicata -loaded Fe nanoparticles, have great cytotoxic effects against LS174t cells, and exposure to radiotherapy just further intensified these results. The in vitro condition revealed alterations in the expression of pro-apoptotic BAX and anti-apoptotic Bcl2, suggesting a pro-apoptotic effect from all three components, particularly the Mentha spicata-loaded Fe nanoparticles. After further clinical trials, these nanoparticles can be used to treat colon cancer.
