Small tandem DNA duplications result from CST-guided Pol α-primase action at DNA break termini

Joost Schimmel; Núria Muñoz-Subirana; Hanneke Kool; Robin van Schendel; Marcel Tijsterman

doi:10.1038/s41467-021-25154-w

Nature Communications (Aug 2021)

Small tandem DNA duplications result from CST-guided Pol α-primase action at DNA break termini

Joost Schimmel,
Núria Muñoz-Subirana,
Hanneke Kool,
Robin van Schendel,
Marcel Tijsterman

Affiliations

Joost Schimmel: Department of Human Genetics, Leiden University Medical Center
Núria Muñoz-Subirana: Department of Human Genetics, Leiden University Medical Center
Hanneke Kool: Department of Human Genetics, Leiden University Medical Center
Robin van Schendel: Department of Human Genetics, Leiden University Medical Center
Marcel Tijsterman: Department of Human Genetics, Leiden University Medical Center

DOI: https://doi.org/10.1038/s41467-021-25154-w
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 11

Abstract

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Error-prone repair of DNA double-strand breaks have been implied to cause cancer-associated genome alterations, but the mechanism of their formation remains unclear. Here the authors find that DNA polymerase α primase plays part in tandem duplication formation at CRISPR/Cas9-induced complementary 3′ ssDNA protrusions.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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