A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution

Abstract

Summary: Innate lymphoid cells (ILCs) and adaptive T cells remain a challenge to study because of a significant overlap in their transcriptomic profiles. Here, we describe the adoptive transfer of ILC progenitors into mice genetically deficient in innate and adaptive immune cells to allow detailed study of the development and function of ILCs and gene regulation in an in vivo setting.For complete details on the use and execution of this protocol, please refer to Jacquelot et al. (2021) and Seillet et al. (2016). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Cell Biology
• Cell isolation
• Flow Cytometry/Mass Cytometry
• Immunology