miRNA-99b-5p targets FZD8 to inhibit non-small cell lung cancer proliferation, migration and invasion

Abstract

Rui Liu,1,2 Yajuan Chen,1 Tao Shou,2 Jing Hu,2 Chen Qing1 1Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650031, People’s Republic of China; 2Department of Oncology, The First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan, People’s Republic of China Background: miRNAs were found to play crucial roles in regulating cellular behaviors. The aim of this study was to investigate the biological function of miRNA-99b-5p (miR-99b-5p) in non-small cell lung cancer (NSCLC). Materials and methods: miR-99b-5p expression level in NSCLC cell lines was detected by quantitative real-time PCR (qRT-PCR). Cell proliferation, migration and invasion were examined by cell counting kit-8 (CCK-8) assay, wound-healing assay and Transwell invasion assay, respectively. Dual-luciferase activity reporter assay and Western blot assay were conducted to validate the target of miR-99b-5p. Results: The expression of miR-99b-5p was decreased in NSCLC cell lines compared with normal cell line. Overexpression of miR-99b-5p inhibits cell proliferation, migration and invasion in vitro. FZD8 was validated as a direct target of miR-99b-5p. Overexpression of FZD8 partially abolished the effects of miR-99b-5p mimic on NSCLC cell behaviors. Conclusion: Collectively, our results demonstrated that miR-99b-5p inhibits NSCLC cell proliferation, migration and invasion through targeting FZD8. This newly identified miR-99b-5p/FZD8 axis provided novel insights into the mechanisms underlying NSCLC progression. Keywords: miR-99b-5p, FZD8, non-small cell lung cancer, migration, invasion

Keywords

• miR-99b-5p
• FZD8
• non-small cell lung cancer
• migration
• invasion