Stem Cells International (Jan 2013)

Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture

  • Masumi Akita,
  • Kayoko Tanaka,
  • Sachiko Matsumoto,
  • Kumiko Komatsu,
  • Keiko Fujita

DOI
https://doi.org/10.1155/2013/927403
Journal volume & issue
Vol. 2013

Abstract

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We detected the hematopoietic stem and progenitor cell marker CD133 using immunogold labeling during angiogenesis in a three-dimensional collagen gel culture. CD133-positive cells were present in capillary tubes newly formed from aortic explants in vitro. The CD133-positive cell population had the capacity to form capillary tubes. Lovastatin strongly inhibited cell migration from aortic explants and caused the degradation of the capillary tubes. The present study provides insight into the function of CD133 during angiogenesis as well as an explanation for the antiangiogenic effect of statins.