Complete Genome Sequence of Bacillus velezensis LQ-3, a Biocontrol Agent Against Rhizoctonia cerealis, Isolated from Wheat Soil

Abstract

Bacillus velezensis LQ-3 was previously isolated from wheat soil and has been found to exhibit strong biocontrol activity against Rhizoctonia cerealis. To better understand its biocontrol mechanism, we utilized both Illumina and Nanopore sequencing technologies to sequence the whole genome of LQ-3. Our analysis revealed that the assembled genome of strain LQ-3 was 3,929,792 bp in length and contained one chromosome with a G+C content of 46.50%. We also identified 3,747 coding sequence genes, 86 tRNA genes, 27 rRNA genes, and one tmRNA gene. The LQ-3 genome contained 12 biosynthetic gene clusters responsible for secondary metabolite production. This genome sequence analysis is crucial for understanding the biocontrol mechanism of B. velezensis LQ-3 and serves as a foundation for the development and industrialization of biocontrol agents. [Figure: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Keywords

• Bacillus
• biocontrol
• genome sequence
• soil
• wheat sharp eyespot