Department of Neurobiology, University of Southern California, Los Angeles, United States; Neuroscience Graduate Program, University of Southern California, Los Angeles, United States
Taylor W Seid
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States; Howard Hughes Medical Institute, California Institute of Technology, Pasadena, United States; Tianqiao and Chrissy Chen Institute for Neuroscience, California Institute of Technology, Pasadena, United States
Research on neuropeptide function has advanced rapidly, yet there is still no spatio-temporally resolved method to measure the release of neuropeptides in vivo. Here we introduce Neuropeptide Release Reporters (NPRRs): novel genetically-encoded sensors with high temporal resolution and genetic specificity. Using the Drosophila larval neuromuscular junction (NMJ) as a model, we provide evidence that NPRRs recapitulate the trafficking and packaging of native neuropeptides, and report stimulation-evoked neuropeptide release events as real-time changes in fluorescence intensity, with sub-second temporal resolution.
