Proteome Science (Dec 2012)

Proteomic analysis of S-nitrosylation induced by 1-methyl-4-phenylpyridinium (MPP<sup>+</sup>)

  • Komatsubara Akira T,
  • Asano Tomoya,
  • Tsumoto Hiroki,
  • Shimizu Kazuharu,
  • Nishiuchi Takumi,
  • Yoshizumi Masanori,
  • Ozawa Kentaro

DOI
https://doi.org/10.1186/1477-5956-10-74
Journal volume & issue
Vol. 10, no. 1
p. 74

Abstract

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Abstract Background Nitric oxide (NO) mediates its function through the direct modification of various cellular targets. S-nitrosylation is a post-translational modification of cysteine residues by NO that regulates protein function. Recently, an imbalance of S-nitrosylation has also been linked to neurodegeneration through the impairment of pro-survival proteins by S-nitrosylation. Results In the present study, we used two-dimensional gel electrophoresis in conjunction with the modified biotin switch assay for protein S-nitrosothiols using resin-assisted capture (SNO-RAC) to identify proteins that are S-nitrosylated more intensively in neuroblastoma cells treated with a mitochondrial complex I inhibitor, 1-methyl-4-phenylpyridinium (MPP+). We identified 14 proteins for which S-nitrosylation was upregulated and seven proteins for which it was downregulated in MPP+-treated neuroblastoma cells. Immunoblot analysis following SNO-RAC confirmed a large increase in the S-nitrosylation of esterase D (ESD), serine-threonine kinase receptor-associated protein (STRAP) and T-complex protein 1 subunit γ (TCP-1 γ) in MPP+-treated neuroblastoma cells, whereas S-nitrosylation of thioredoxin domain-containing protein 5 precursor (ERp46) was decreased. Conclusions These results suggest that S-nitrosylation resulting from mitochondrial dysfunction can compromise neuronal survival through altering multiple signal transduction pathways and might be a potential therapeutic target for neurodegenerative diseases.